3ZW8
Crystal Structure Of Rat Peroxisomal Multifunctional Enzyme Type 1 (rpMFE1) In Apo Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-26 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.220, 125.680, 226.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.500 |
| R-factor | 0.2324 |
| Rwork | 0.230 |
| R-free | 0.27673 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x58 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.621 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.130 | 0.220 |
| Number of reflections | 56678 | |
| <I/σ(I)> | 8.9 | 5.4 |
| Completeness [%] | 86.8 | 73.6 |
| Redundancy | 5.5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 100MM MES PH 6.0, 150MM AMMONIUM SULPHATE, 15% W/V PEG4000 |
