3ZW7
The 3-dimensional structure of MpgP from Thermus thermophilus HB27, in complex with the alpha-mannosylglycerate and metaphosphate.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-03 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.794, 70.804, 92.770 |
Unit cell angles | 90.00, 95.54, 90.00 |
Refinement procedure
Resolution | 24.958 - 1.898 |
R-factor | 0.1644 |
Rwork | 0.162 |
R-free | 0.20340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zty |
RMSD bond length | 0.007 |
RMSD bond angle | 1.009 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.010 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.030 | 0.260 |
Number of reflections | 39808 | |
<I/σ(I)> | 23.79 | 4.92 |
Completeness [%] | 98.0 | 97.3 |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | SEE ACTAF REFERENCE IN REMARK 1 ABOVE., pH 6.5 |