3ZW2
Structure of the lectin Bambl from Burkholderia ambifaria in complex with blood group H type 1 tetrasaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-07 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.640, 47.570, 72.940 |
| Unit cell angles | 90.00, 103.19, 90.00 |
Refinement procedure
| Resolution | 39.520 - 1.600 |
| R-factor | 0.16216 |
| Rwork | 0.160 |
| R-free | 0.20235 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zw0 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.681 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.520 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.070 | 0.180 |
| Number of reflections | 29207 | |
| <I/σ(I)> | 7.1 | 2.5 |
| Completeness [%] | 95.3 | 78.9 |
| Redundancy | 2.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 3 M MALONATE PH 6.0. 10% ETHYLENE GLYCOL WAS ADDED AS CRYOPROTECTANT PRIOR FREEZING |
