3ZVH
Methylaspartate ammonia lyase from Clostridium tetanomorphum mutant Q73A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-10 |
| Detector | MAC Science DIP-2030 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.788, 109.705, 110.096 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.710 - 1.990 |
| R-factor | 0.19768 |
| Rwork | 0.195 |
| R-free | 0.24889 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kcz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.324 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.160 | 0.580 |
| Number of reflections | 54091 | |
| <I/σ(I)> | 6.9 | 2.1 |
| Completeness [%] | 98.0 | 95.4 |
| Redundancy | 4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 14-17 % PEG6000, MES PH 6.0, 100 MM MGCL2 |
