3ZUT

The structure of OST1 (D160A) kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-06
Detector	ADSC QUANTUM 210
Spacegroup name	P 2 2 21
Unit cell lengths	77.273, 99.195, 107.832
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.740 - 2.500
R-factor	0.2002
Rwork	0.198
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hyh
RMSD bond length	0.010
RMSD bond angle	1.078
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.740	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.060	0.520
Number of reflections	29227
<I/σ(I)>	13.3	2.8
Completeness [%]	99.7	99.7
Redundancy	4.5	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M HEPES PH 6.5, 12% PEG10000 AND 12% ETHYLENE GLYCOL.