3ZUM
Photosynthetic Reaction Centre Mutant with Phe L146 replaced with Ala
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS |
| Synchrotron site | SRS |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4r |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 139.461, 139.461, 185.014 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 119.520 - 2.500 |
| R-factor | 0.19694 |
| Rwork | 0.195 |
| R-free | 0.22786 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.030 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 119.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.080 |
| Number of reflections | 68081 |
| Completeness [%] | 99.7 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | VAPOUR DIFFUSION, SITTING DROP, 0.09% LDAO, 3.5% 1,2,3 HEPTANETRIOL, 0.75 M POTASSIUM PHOSPHATE PH 7.5 |
