3ZU0

Structure of Haemophilus influenzae NAD nucleotidase (NadN)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	101.626, 104.051, 119.601
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.707 - 2.001
R-factor	0.215
Rwork	0.215
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z1a
RMSD bond length	0.008
RMSD bond angle	1.344
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.070	0.310
Number of reflections	83213
<I/σ(I)>	8.2	3
Completeness [%]	97.0	95.6
Redundancy	3.1	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1