3ZTY
The 3-dimensional structure of the gadolinium derivative of MpgP, the mannosyl-3-phosphoglycerate phosphatase from Thermus thermophilus HB27
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-21 |
| Detector | Bruker Platinum 135 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.692, 70.835, 92.735 |
| Unit cell angles | 90.00, 95.04, 90.00 |
Refinement procedure
| Resolution | 37.561 - 2.500 |
| R-factor | 0.1983 |
| Rwork | 0.195 |
| R-free | 0.25260 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.120 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | SHELX (CDE) |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.500 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.360 |
| Number of reflections | 17882 | |
| <I/σ(I)> | 17.2 | 4.7 |
| Completeness [%] | 99.7 | 98.2 |
| Redundancy | 10.9 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | SEE REMARK 1 REFERENCE ABOVE., pH 6.5 |
