3ZTW
The 3-dimensional structure of apo-MpgP, the mannosyl-3- phosphoglycerate phosphatase from Thermus thermophilus HB27 in its apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-14 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.520, 70.686, 95.422 |
| Unit cell angles | 90.00, 92.95, 90.00 |
Refinement procedure
| Resolution | 39.510 - 1.898 |
| R-factor | 0.1757 |
| Rwork | 0.173 |
| R-free | 0.22810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zty |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.965 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | 0.480 |
| Number of reflections | 40742 | |
| <I/σ(I)> | 18.8 | 2.4 |
| Completeness [%] | 98.0 | 88.6 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | SEE REMARK 1 REFERENCE, pH 6.5 |
