3ZTL
Crystal structure of decameric form of Peroxiredoxin I from Schistosoma mansoni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.507, 116.904, 117.349 |
| Unit cell angles | 69.12, 92.00, 86.93 |
Refinement procedure
| Resolution | 43.002 - 3.000 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.24780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pn8 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.953 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.460 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.150 | 0.470 |
| Number of reflections | 49104 | |
| <I/σ(I)> | 9 | 3.2 |
| Completeness [%] | 98.9 | 98.7 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG3350, 0.02M DTT, 0.2M TRI-POTASSIUM CITRATE |
