3ZTF
X-ray Structure of the Cyan Fluorescent Protein mTurquoise2 (K206A mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.010, 62.549, 70.356 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.180 - 1.310 |
| R-factor | 0.13429 |
| Rwork | 0.132 |
| R-free | 0.17737 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ye0 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.705 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.700 | 1.380 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Rmerge | 0.060 | 0.700 |
| Number of reflections | 52472 | |
| <I/σ(I)> | 10.8 | 2 |
| Completeness [%] | 96.1 | 94.6 |
| Redundancy | 4.2 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.25 | 30 MG/ML PROTEIN, 19% PEG 8000, 100 MM MGCL2, 100 MM HEPES PH 7.25 . |
