3ZTB
The bacterial stressosome: a modular system that has been adapted to control secondary messenger signaling
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-24 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.537, 60.976, 88.954 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.815 - 2.800 |
| R-factor | 0.2137 |
| Rwork | 0.210 |
| R-free | 0.27320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vy9 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.908 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.000 | 3.520 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.060 | 0.190 |
| Number of reflections | 7170 | |
| <I/σ(I)> | 20.1 | 8.7 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 6.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1 UL PROTEIN AND 1 UL CRYSTALLANT, 1 ML WELL, 20 % PEG 3350, 0.2 M NAI, 0.1 M BIS-TRIS, PH 6.5. |
