3ZT6
GlgE isoform 1 from Streptomyces coelicolor with alpha-cyclodextrin and maltose bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 113.850, 114.160, 315.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.390 - 2.190 |
| R-factor | 0.20949 |
| Rwork | 0.208 |
| R-free | 0.23625 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zst |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.455 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0101) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.990 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.150 | 0.600 |
| Number of reflections | 206349 | |
| <I/σ(I)> | 7.7 | 2.1 |
| Completeness [%] | 98.9 | 93.2 |
| Redundancy | 5.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.0 |
