3ZS8
S. cerevisiae Get3 complexed with a cytosolic Get1 fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 110.276, 110.276, 315.956 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.215 - 3.000 |
| R-factor | 0.2261 |
| Rwork | 0.220 |
| R-free | 0.28070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h84 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.830 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.090 | 0.730 |
| Number of reflections | 23431 | |
| <I/σ(I)> | 24.1 | 1.7 |
| Completeness [%] | 98.6 | 87.3 |
| Redundancy | 8.2 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 0.2 M K/NA TARTRATE, 16% PEG3350, 0.1 M HEPES, PH 7.2 AND 6% POLYPROPYLENE GLYCOL P400 |
