3ZS7
Crystal structure of pyridoxal kinase from Trypanosoma brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-05-05 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.210, 80.000, 106.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.710 - 2.000 |
| R-factor | 0.22171 |
| Rwork | 0.219 |
| R-free | 0.26537 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yxt |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.307 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.320 |
| Number of reflections | 18739 | |
| <I/σ(I)> | 23.7 | 5.1 |
| Completeness [%] | 92.0 | 84.7 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
