3ZS4
CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS PHOSPHORIBOSYL ISOMERASE WITH BOUND PRFAR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 63.600, 63.600, 132.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.350 - 1.900 |
| R-factor | 0.17632 |
| Rwork | 0.174 |
| R-free | 0.21374 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2BNT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.103 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.350 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.510 |
| Number of reflections | 22159 | |
| <I/σ(I)> | 17.63 | 4.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.5 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS-HCL PH 8.5, 2.4 M DI-AMMONIUM HYDROGEN PHOSPHATE |
