3ZRY

Rotor architecture in the F(1)-c(10)-ring complex of the yeast F-ATP synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-07
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	107.690, 174.910, 164.260
Unit cell angles	90.00, 93.92, 90.00

Refinement procedure

Resolution	54.625 - 6.500
R-factor	0.3178
Rwork	0.317
R-free	0.33870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xok
RMSD bond length	0.003
RMSD bond angle	0.944
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.620	6.850
High resolution limit [Å]	6.500	6.500
Rmerge	0.120	0.920
Number of reflections	11687
<I/σ(I)>	8.9	1.8
Completeness [%]	96.6	97.2
Redundancy	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		10% PEG 4000, 100 MM SODIUM CHLORIDE, 100 MM HEPES PH 6.5 MIXED 1:1 WITH PROTEIN SOLUTION (10 MG/ML) CONTAINING 0.64 MM DDM, 25 MM TRIS PH 8.0, 100 MM SODIUM CHLORIDE, 25 MM TREHALOSE, 0.5 MM EDTA, 3 MM SODIUM AZIDE, 2 MM MAGNESIUM CHLORIDE, 0.04 MM ADP, 1 MM AMP-PNP, 0.1 MM DCCD, 2.5 MM DTT, 0.5 MM PMSF.