3ZRW
The structure of the dimeric Hamp-Dhp fusion A291V mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH MX-225 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.860, 46.670, 157.460 |
| Unit cell angles | 90.00, 94.31, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.250 |
| R-factor | 0.21696 |
| Rwork | 0.214 |
| R-free | 0.26946 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2asm |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.985 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.090 | 0.530 |
| Number of reflections | 23404 | |
| <I/σ(I)> | 12 | 2.6 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.4 M MAGNESIUM FORMATE, 0.1 M BIS/TRIS, PH 7. |
