3ZRS
X-ray crystal structure of a KirBac potassium channel highlights a mechanism of channel opening at the bundle-crossing gate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 106.240, 106.240, 89.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.800 - 3.050 |
| R-factor | 0.2222 |
| Rwork | 0.220 |
| R-free | 0.25890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x6c |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.240 | 3.210 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.130 | 0.940 |
| Number of reflections | 10293 | |
| <I/σ(I)> | 5.5 | 2 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.8 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% GLYCEROL, 90 MM HEPES 7.2, 20.0% PEG 400, 5% PEG 4K, 2.5% PEG 8K. |
