3ZRR
Crystal structure and substrate specificity of a thermophilic archaeal serine : pyruvate aminotransferase from Sulfolobus solfataricus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 345 mm plate |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 160.835, 55.205, 102.320 |
Unit cell angles | 90.00, 111.95, 90.00 |
Refinement procedure
Resolution | 94.920 - 1.990 |
R-factor | 0.18865 |
Rwork | 0.186 |
R-free | 0.23135 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.301 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0062) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.090 | 0.510 |
Number of reflections | 51737 | |
<I/σ(I)> | 10.4 | 2 |
Completeness [%] | 95.9 | 91.9 |
Redundancy | 2.8 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7 |