3ZRP
Crystal structure and substrate specificity of a thermophilic archaeal serine : pyruvate aminotransferase from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.430, 54.970, 101.810 |
| Unit cell angles | 90.00, 111.27, 90.00 |
Refinement procedure
| Resolution | 94.870 - 1.750 |
| R-factor | 0.21827 |
| Rwork | 0.216 |
| R-free | 0.25960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bkw |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.291 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.6.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.050 | 0.390 |
| Number of reflections | 76065 | |
| <I/σ(I)> | 8.7 | 1.6 |
| Completeness [%] | 95.0 | 76.5 |
| Redundancy | 2.7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 5 MG/ML PROTEIN, 50 MM TRIS HCL PH 8.5 AND 15% POLYETHYLENE GLYCOL (PEG) 400. |
