3ZRJ

Complex of ClpV N-domain with VipB peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-17
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	124.240, 37.610, 88.130
Unit cell angles	90.00, 107.54, 90.00

Refinement procedure

Resolution	42.700 - 1.940
Rwork	0.183
R-free	0.22500
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.023
RMSD bond angle	1.836
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	2.000
High resolution limit [Å]	1.940	1.940
Rmerge	0.060
Number of reflections	28218
<I/σ(I)>	14.4	3.7
Completeness [%]	96.4	91.2
Redundancy	3.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			277	0.1M MES PH 5.5, 17.5% (W/V) PEG 8000, 4C