3ZR8

Crystal structure of RxLR effector Avr3a11 from Phytophthora capsici

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	21.300, 25.330, 90.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.410 - 0.900
R-factor	0.12669
Rwork	0.126
R-free	0.14865
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.017
RMSD bond angle	2.371
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	17.410
High resolution limit [Å]	0.900
Rmerge	0.060
Number of reflections	34450
<I/σ(I)>	14
Completeness [%]	97.5
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				25-30% (W/V) PEG 3350, 0.1 M BIS-TRIS PROPANE PH 6.5-7.0, 0.2 M POTASSIUM THIOCYANATE.