3ZQQ
Crystal structure of the full-length small terminase from a SPP1-like bacteriophage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-04 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 98.184, 98.184, 323.818 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 107.000 - 4.000 |
| R-factor | 0.1996 |
| Rwork | 0.200 |
| R-free | 0.28220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2CMP AND 3ZQP |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.660 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.000 | 4.230 |
| High resolution limit [Å] | 4.010 | 4.010 |
| Rmerge | 0.080 | 0.190 |
| Number of reflections | 3308 | |
| <I/σ(I)> | 6.6 | 4.1 |
| Completeness [%] | 62.0 | 18 |
| Redundancy | 8.5 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M MGCL2, 8 % PEG 2000, 8 % PEG 550MME, 0.1 M TRIS PH 8.0 |
