3ZQO
Crystal structure of the small terminase oligomerization core domain from a SPP1-like bacteriophage (crystal form 3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.202, 198.201, 59.202 |
| Unit cell angles | 90.00, 90.81, 90.00 |
Refinement procedure
| Resolution | 22.100 - 1.680 |
| R-factor | 0.18339 |
| Rwork | 0.183 |
| R-free | 0.23357 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zqm |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.417 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.100 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.100 | 0.330 |
| Number of reflections | 127549 | |
| <I/σ(I)> | 8 | 3.1 |
| Completeness [%] | 85.6 | 98 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.7 M (NH4)2SO4, 0.2 M TRIS PH 8.5 |
