3ZQM
Crystal structure of the small terminase oligomerization core domain from a SPP1-like bacteriophage (crystal form 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.954, 60.009, 81.442 |
| Unit cell angles | 90.00, 100.43, 90.00 |
Refinement procedure
| Resolution | 80.060 - 1.850 |
| R-factor | 0.17389 |
| Rwork | 0.172 |
| R-free | 0.21351 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.581 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.080 | 0.360 |
| Number of reflections | 52842 | |
| <I/σ(I)> | 18 | 4.3 |
| Completeness [%] | 98.0 | 86 |
| Redundancy | 4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M MALONATE/IMIDAZOLE/BORIC ACID BUFFER SYSTEM PH 7.0, 25 % PEG 1500 |
