Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 258.227, 258.227, 204.554 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.400 |
| R-factor | 0.27336 |
| Rwork | 0.273 |
| R-free | 0.32422 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r6f |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.761 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.090 | 0.430 |
| Number of reflections | 106710 | |
| <I/σ(I)> | 8.2 | 2 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1.5 M AMMONIUM SULFATE, 0.1 M HEPES PH 7.5, 1 MM YTTRIUM CHLORIDE. |
