3ZQ7
The Structure of DNA-binding domain of response regulator from Escherichia coli K-12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-07-22 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 36.440, 36.440, 138.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.240 - 2.520 |
| R-factor | 0.24079 |
| Rwork | 0.236 |
| R-free | 0.28307 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oqr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.664 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 35.000 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.100 |
| Number of reflections | 3378 | |
| <I/σ(I)> | 19 | 9.1 |
| Completeness [%] | 95.0 | 77.7 |
| Redundancy | 4.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | LITHIUM SULFATE, 0.1M HEPES PH 7.5. |
