3ZQ3
Crystal Structure of Rat Odorant Binding Protein 3 (OBP3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.980, 100.530, 72.410 |
| Unit cell angles | 90.00, 110.61, 90.00 |
Refinement procedure
| Resolution | 67.776 - 2.800 |
| R-factor | 0.219 |
| Rwork | 0.218 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a2u |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.371 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.800 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.130 | 0.480 |
| Number of reflections | 19952 | |
| <I/σ(I)> | 4.8 | 1.8 |
| Completeness [%] | 95.7 | 95.1 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.02 M D-GLUCOSE, 0.02 M D-GALACTOSE, 0.02 M L-FUCOSE, 0.02M D-XYLOSE, 0.02M N-ACETYL-D-GLUCOSAMINE, 0.1 M MORPHEUS BUFFER 1 PH 6.5, 10 % W/V POLYETHYLENE GLYCOL, 20 % POLYETHYLENE GLYCOL 550 |
