3ZPX
USTILAGO MAYDIS LIPASE UM03410, SHORT FORM WITHOUT FLAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.753, 100.150, 129.158 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.570 - 1.990 |
| R-factor | 0.18539 |
| Rwork | 0.183 |
| R-free | 0.23469 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3guu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.241 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.110 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.080 | 0.510 |
| Number of reflections | 47507 | |
| <I/σ(I)> | 11.3 | 2.4 |
| Completeness [%] | 95.6 | 90 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 30% PEG 8000, 100 MM NAOAC PH 4.5, 200 MM LI2SO4. CRYO SOLUTION: 20% PEG 8000, 20% PEG 400 |
