3ZPV
Crystal structure of Drosophila Pygo PHD finger in complex with Legless HD1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.210, 111.960, 190.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.240 - 2.680 |
| R-factor | 0.22582 |
| Rwork | 0.224 |
| R-free | 0.26231 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vp7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.597 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0024) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.180 | 2.820 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.140 | 0.610 |
| Number of reflections | 63722 | |
| <I/σ(I)> | 8.7 | 2.2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.136 M (NH4)2SO4, 100 MM TRIS PH 8.3, 200 MM NACL |
