3ZPL
Crystal structure of Sco3205, a MarR family transcriptional regulator from Streptomyces coelicolor, in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 |
| Unit cell lengths | 70.800, 70.800, 557.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.880 - 2.800 |
| R-factor | 0.17668 |
| Rwork | 0.176 |
| R-free | 0.19649 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zmd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.351 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.680 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.370 |
| Number of reflections | 37192 | |
| <I/σ(I)> | 13.7 | 1.9 |
| Completeness [%] | 96.0 | 78.2 |
| Redundancy | 8.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 20-40% (V/V) MPD, 80-100 MM SODIUM ACETATE AND 50 MM MES PH 5.6 |
