3ZPJ
Crystal structure of Ton1535 from Thermococcus onnurineus NA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 295 |
| Detector technology | CCD |
| Collection date | 2011-06-14 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 117.954, 117.954, 208.232 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.911 - 2.304 |
| R-factor | 0.1969 |
| Rwork | 0.194 |
| R-free | 0.24590 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.139 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.390 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.280 |
| Number of reflections | 24870 | |
| <I/σ(I)> | 45.5 | 9.7 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 9.1 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M BIS-TRIS PROPANE (PH7.0), 1.2 M POTASSIUM SODIUM TARTRATE TETRAHYDRATE |
