3ZOZ
The structure of human phosphoglycerate kinase with bound bromide, a stimulating anion.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.980, 91.810, 108.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.16382 |
| Rwork | 0.161 |
| R-free | 0.20878 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wzc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.414 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.080 | 0.250 |
| Number of reflections | 29204 | |
| <I/σ(I)> | 16.7 | 7 |
| Completeness [%] | 99.2 | 99.4 |
| Redundancy | 8.6 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 35% PEG2000MME, 0.1M BIS-TRIS PH 6.5 |
