3ZOX
Crystal Structure of N64Del Mutant of Nitrosomonas europaea Cytochrome c552 (monoclinic space group)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-04 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.520, 81.240, 42.280 |
| Unit cell angles | 90.00, 105.17, 90.00 |
Refinement procedure
| Resolution | 40.840 - 2.100 |
| R-factor | 0.2152 |
| Rwork | 0.212 |
| R-free | 0.27440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 351c |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.983 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.800 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.150 | 0.500 |
| Number of reflections | 24887 | |
| <I/σ(I)> | 4.7 | 2.3 |
| Completeness [%] | 94.3 | 94 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 0.1 M CHES PH 9.5, 30% (W/V) PEG 8000 |
