3ZOU
Native structure of Farnesyl Pyrophosphate Synthase from Pseudomonas aeruginosa PA01, with bound fragment SPB02696, and substrate geranyl pyrophosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-09 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 85.040, 98.640, 130.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.690 - 1.550 |
| R-factor | 0.16335 |
| Rwork | 0.162 |
| R-free | 0.19541 |
| Structure solution method | SIR |
| Starting model (for MR) | 3zcd |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.894 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.670 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.060 | 0.540 |
| Number of reflections | 77089 | |
| <I/σ(I)> | 13.1 | 1.9 |
| Completeness [%] | 96.9 | 75.8 |
| Redundancy | 4.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 15% GLYCEROL, 0.2 M MGCL2, 20% PEG6000, 0.1 M TRIS PH 8, 0.274 MM GPP, 10 MM SPB02696, 10% METHANOL, 1% DMSO |
