3ZOT
Structure of E.coli rhomboid protease GlpG in complex with monobactam L29 (data set 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-11 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 110.200, 110.200, 128.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.379 - 2.399 |
| R-factor | 0.2008 |
| Rwork | 0.198 |
| R-free | 0.25170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xov |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.061 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.400 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.070 | 0.460 |
| Number of reflections | 11901 | |
| <I/σ(I)> | 12.6 | 3.1 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 4.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.25M NH4CL, 0.1M BIS-TRIS, PH7.0, 298K |
