3ZOQ
Structure of BsUDG-p56 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.770, 66.270, 102.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.620 - 1.450 |
| R-factor | 0.14146 |
| Rwork | 0.139 |
| R-free | 0.18157 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eug |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.167 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.860 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.070 | 0.380 |
| Number of reflections | 65433 | |
| <I/σ(I)> | 16.4 | 4.6 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 8 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 277 | 19% PEG 8000, 0.1 M TRIS-HCL, PH 8.5, 0.25 M MAGNESIUM CHLORIDE AT 277 K |
