3ZOE
Crystal structure of FMN-binding protein (YP_005476) from Thermus thermophilus with bound p-hydroxybenzaldehyde
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-30 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.660, 74.910, 77.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.460 - 1.850 |
| R-factor | 0.24381 |
| Rwork | 0.242 |
| R-free | 0.27771 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1usc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.755 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.500 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | 0.480 |
| Number of reflections | 39042 | |
| <I/σ(I)> | 16 | 3.3 |
| Completeness [%] | 92.7 | 96 |
| Redundancy | 5.9 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MORPHEUS SCREEN H5 10% W/V PEG 20 000, 20% V/V PEG MME 550; 0.02 M OF EACH AMINO ACID; 0.1 M MOPS/HEPES-NA PH 7.5 |
