3ZO8
Wild-type chorismate mutase of Bacillus subtilis at 1.6 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-11-02 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.498, 165.174, 49.586 |
| Unit cell angles | 90.00, 118.67, 90.00 |
Refinement procedure
| Resolution | 82.600 - 1.590 |
| R-factor | 0.1452 |
| Rwork | 0.144 |
| R-free | 0.16975 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dbf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.171 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (FOR MR) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.580 | 1.690 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.050 | 0.180 |
| Number of reflections | 93148 | |
| <I/σ(I)> | 14.4 | 4.4 |
| Completeness [%] | 85.0 | 59.4 |
| Redundancy | 2.9 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 100 MM MMT BUFFER ---MALIC ACID, MES, TRIS, IN MOLAR RATIOS OF 1:2:2, RESPECTIVELY--- PH 6.0, 100 MM MAGNESIUM CHLORIDE, 25% W/V PEG 1000 |
