3ZNH
Crimean Congo Hemorrhagic Fever Virus OTU domain in complex with ubiquitin-propargyl.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 146.050, 146.050, 58.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 126.480 - 2.300 |
| R-factor | 0.21385 |
| Rwork | 0.211 |
| R-free | 0.27465 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3phw |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.029 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.120 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.690 |
| Number of reflections | 16479 | |
| <I/σ(I)> | 17.6 | 3.6 |
| Completeness [%] | 98.6 | 97.4 |
| Redundancy | 4.6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 20-30% PEG 8000, 100 MM NA CACODYLATE PH 6.5, 100 MM MG ACETATE, AND 2% N-OCTYL-BETA-D-GLUCOSIDE. |
