3ZNG
Ankyrin repeat and SOCS-box protein 9 (ASB9) in complex with ElonginB and ElonginC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 114.110, 103.130, 109.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.638 - 2.850 |
| R-factor | 0.1969 |
| Rwork | 0.195 |
| R-free | 0.24160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zkj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.185 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.640 | 2.920 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.120 | 0.910 |
| Number of reflections | 30887 | |
| <I/σ(I)> | 14.2 | 2.8 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 11.4 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BIS-TRIS PROPANE, PH 6.7, 0.2 M SODIUM MALONATE, 22% PEG 3350, 10% ETHYLENE GLYCOL |
