3ZNB
METALLO-BETA-LACTAMASE (ZN, HG-BOUND FORM)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 130 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-03-22 |
| Detector | SIEMENS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.200, 78.200, 140.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.26800 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 1ZNB WITHOUT METALS OR SOLVENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.800 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.870 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.045 * | |
| Number of reflections | 11371 | |
| <I/σ(I)> | 23 | 6.4 |
| Completeness [%] | 90.0 | 47 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | Concha, N.O., (1996) Structure (London), 4, 823. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-8 (mg/ml) | |
| 2 | 1 | drop | HEPES | 55 (mM) | |
| 3 | 1 | drop | 0.01 (mM) | ||
| 4 | 1 | drop | PEG2000 | 26-28 (%) | |
| 5 | 1 | reservoir | PEG2000 | 26-28 (%) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) |
