3ZMJ
Structure of E.coli rhomboid protease GlpG in complex with monobactam L61
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-07 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 110.280, 110.280, 128.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.235 - 2.300 |
| R-factor | 0.1981 |
| Rwork | 0.197 |
| R-free | 0.23060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xov |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.036 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_1284)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.240 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.590 |
| Number of reflections | 13419 | |
| <I/σ(I)> | 14.4 | 3 |
| Completeness [%] | 99.2 | 99.9 |
| Redundancy | 5.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.75 M AMMONIUM CHLORIDE, 0.1M BIS-TRIS, PH 7.0, 298 K |
