3ZMB
Native structure of Farnesyl Pyrophosphate Synthase from Pseudomonas aeruginosa PA01, with bound fragment SPB02696.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-10 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 85.560, 98.840, 131.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.900 - 1.900 |
R-factor | 0.19114 |
Rwork | 0.188 |
R-free | 0.24095 |
Structure solution method | SIR |
Starting model (for MR) | 3zcd |
RMSD bond length | 0.016 |
RMSD bond angle | 1.662 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.880 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.120 | 0.660 |
Number of reflections | 43864 | |
<I/σ(I)> | 7.1 | 1.9 |
Completeness [%] | 99.3 | 99.5 |
Redundancy | 3.7 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 0.2 M MGCL2, 20% PEG6000, 0.1 M TRIS PH 8 |