3ZLZ
Lys6-linked tri-ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-11-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 104.930, 104.930, 104.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.182 - 2.900 |
| R-factor | 0.2542 |
| Rwork | 0.249 |
| R-free | 0.28820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w9n |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.560 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.200 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.130 | 0.680 |
| Number of reflections | 4579 | |
| <I/σ(I)> | 7.4 | 2.2 |
| Completeness [%] | 97.2 | 99.3 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 20% (V/V) PEG3350 AND 0.2 M ZINC ACETATE, pH 7.4 |
