3ZLG
Structure of group A Streptococcal enolase K362A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-31 |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | P 4 |
| Unit cell lengths | 187.255, 187.255, 57.156 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.969 - 2.100 |
| R-factor | 0.1834 |
| Rwork | 0.179 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w6t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.926 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.970 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.150 | 1.500 |
| Number of reflections | 116292 | |
| <I/σ(I)> | 14.16 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 1-3.5 M SODIUM/POTASSIUM PHOSPHATE BUFFER (PH 5-7.5); 2% PEG 400 |
