3ZLA
Crystal structure of the nucleocapsid protein from Bunyamwera virus bound to RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-10 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 382.240, 88.724, 88.790 |
| Unit cell angles | 90.00, 94.30, 90.00 |
Refinement procedure
| Resolution | 88.700 - 3.200 |
| R-factor | 0.24939 |
| Rwork | 0.247 |
| R-free | 0.29676 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SBV NUCLEOCAPSID PROTEIN |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.327 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 190.580 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.050 | 0.590 |
| Number of reflections | 47586 | |
| <I/σ(I)> | 13.2 | 1.9 |
| Completeness [%] | 96.9 | 99 |
| Redundancy | 3.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.7 | CRYSTALLISATION CONDITION: 11% PEG6000, 0.1 M SODIUM CITRATE PH 5.7, 4 M NACL, 4 MM ZINC ACETATE. CRYO CONDITION: 10% GLYCEROL. |
