3ZL9
Crystal structure of the nucleocapsid protein from Schmallenberg virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-28 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 32 |
| Unit cell lengths | 81.030, 81.030, 128.551 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.590 - 2.750 |
| R-factor | 0.16601 |
| Rwork | 0.164 |
| R-free | 0.20129 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SBV NUCLEOCAPSID PROTEIN |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.291 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.590 | 2.900 |
| High resolution limit [Å] | 3.200 | 2.750 |
| Rmerge | 0.080 | 0.560 |
| Number of reflections | 24459 | |
| <I/σ(I)> | 8.7 | 2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | CRYSTALLISATION CONDITION: 17% PEG10000, 100 MM BIS-TRIS PH 5.5, 100 MM AMMONIUM ACETATE. CRYO CONDITION: 20% GLYCEROL |
