3ZKB

CRYSTAL STRUCTURE OF THE ATPASE REGION OF Mycobacterium tuberculosis GyrB WITH AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Spacegroup name	P 1
Unit cell lengths	101.360, 138.200, 147.690
Unit cell angles	105.28, 92.31, 107.23

Refinement procedure

Resolution	24.940 - 2.900
R-factor	0.1845
Rwork	0.182
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER (2.11.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.050	0.420
Number of reflections	159199
<I/σ(I)>	14.1	1.9
Completeness [%]	98.3	97.7
Redundancy	1.91

Crystallization Conditions

crystal ID	method	pH	temperature	details
1